Biotechnology & Pharmaceuticals
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The value of magnetic resonance arthrography of the temporomandibular joint in imaging disc adhesions and perforations
This study attempted to validate MR arthrography (MRAr) of the temporomandibular joint (TMJ) in detecting the position, integrity and relations of the articular disc and retrodiscal tissue. Temporomandibular joint dysfunction (TMD) is a common disorder affecting more than 5% of the general population.1 Many patients show only mild signs and symptoms and only a fraction of the above percentage come to the physician’s attention. A variety of causative and exacerbating factors are involved and interact in the pathogenesis and clinical course of TMD.
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Chemometric Algorithms for the Direct Determination of Lipids in Synthetic Mixtures and Human Serum
Biotech Research Journals: Although polyunsaturated fatty acids (PUFAs) can be detected by chromatographic methods, a low cost method to quantify PUFAs in serum samples with little to no separation or sample preparation would enable PUFAs to be monitored and detected during routine clinical serum analysis. The goal of this research project was to develop a simple, direct alternative method for the determination of the PUFAs in addition to cholesterol in human serum. The simple colorimetric assay used is rapid, rugged, inexpensive, and specific to the -C=CH-CH2- group that accomplishes, in a single assay the simultaneous quantitation of cholesterol, ?-3 (methyl esters of linolenic, eicosapentaenoic (EPA) and docosahexaenoic (DHA)) fatty acids, and ?-6 (methyl esters of linoleic, conjugated linoleic (CLA), and arachidonic) fatty acids.
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An Efficient Enrichment Technique for Isolation and Quantification of Indigenous Diesel Fuel-utilizing Bacteria
Biotechnology research journals : An efficient enrichment technique for isolation and quantification of indigenous diesel fuel-utilizing bacteria present in freshwater sediments. The toxic effects of petroleum products on the environment, particularly diesel fuel, have been well studied. Equally well researched are the methods for efficient bioremediation of petroleum pollution. Many studies have demonstrated the ability of indigenous microbiota, found in petroleum-polluted environments, to biodegrade diesel fuel. In this study, we quantified the diesel fuel-utilizing bacteria in sediments from two freshwater lakes and then began an enrichment process using a regimen of constant aeration, addition of (NH4)3HPO4 and diesel fuel. The enrichment process was performed in a tabletop bioremediation station based on an Eastern European ship-based bioremediation system.
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Comparison of the Virulence Factors and Analysis of Hypothetical Sequences
Computer Science & Systems Biology Journal: Comparison of the Virulence Factors and Analysis of Hypothetical Sequences of the Strains TIGR4, D39, G54 and R6 of Streptococcus Pneumoniae.
Whole genome sequences of the four strains of Streptococcus pneumoniae, encapsulated TIGR4, D39, G54 and nonencapsulated R6 are considered for the comparative study on genome features, whole genome pairwise alignment, gene role category, and virulence factors using relevant comparative genomics tools. The study of capsular polysaccharide synthesizing genes reveals that many cps genes are unique to TIGR4, which shows the high virulence nature of TIGR4. Further, the study on the other virulence factors such as pneumococcal surface protein A, autolysin, hyaluronate lyase, pneumolysin, neuraminidase B, and pneumococcal surface antigen A of TIGR4 are much related to those of the other three strains, and hence the virulence nature due to these factors among four strains seems to be similar.
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Journal of Management of High-Throughput DNA Sequencing Projects: Alpheus
High-throughput DNA sequencing has enabled systems biology to begin to address areas in health, agricultural and basic biological research. Concomitant with the opportunities is an absolute necessity to manage significant volumes of high-dimensional and interrelated data and analysis. Alpheus is an analysis pipeline, database and visualization software for use with massively parallel DNA sequencing technologies that feature multigigabase throughput characterized by relatively short reads, such as Illumina-Solexa (sequencing-by-synthesis), Roche454 (pyrosequencing) and Applied Biosystem’s SOLiD (sequencing-by-ligation). Alpheus enables alignment to reference sequence(s), detection of variants and enumeration of sequence abundance, including expression levels in transcriptome sequence.
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Clinical and Economic Considerations in The Use of Inhaled Anesthesia from The Perspective of Health-System Pharmacists and Anesthesiologists
Articles in Journal based on the proceedings of a symposium held December 8, 2009, during the 44th ASHP Midyear Clinical Meeting and Exhibition in Las Vegas, Nevada. The content of this supplement was written by a professional writer, Susan R. Dombrowski, M.S., and was reviewed, revised, and approved by the authors. Ms. Dombrowski reports no affiliation with or financial interest in a commercial organization that poses a confict or interest with this supplement. The activity was supported by an educational grant from Baxter Healthcare Corporation.
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Nuclear Power Development: International Trade & Waste and Fuel Management
Nuclear cooperation between different nations has been an element of nuclear power development and operation since the earliest days of the nuclear power. Nuclear cooperation has taken different forms and has served a variety of purposes.
Contents: Developments in International Nuclear Cooperation, Reviving American Nuclear Industry, An Opportunity for US to Lead by Example, Supporting Advanced Fuel Technologies, Nuclear Power for Process Heat Applications, $2.38 Million Savings with Simple Innovation, $50 Million/Plant Savings with
IN-SITU Epoxy Coating, Improved Service Water Performance with Polyethylene Piping, Supporting 1,000 Well-Paying Jobs.
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Application of biorelevant dissolution tests to the prediction of in vivo performance
Pharmaceutical Sciences Journals : Application of biorelevant dissolution tests to the prediction of in vivo performance of diclofenac sodium from an oral modified-release pellet dosage form. In vitro biorelevant dissolution tests enabling the prediction of in vivo performance of an oral modifiedrelease (MR) dosage formwere developed in this study. In vitro dissolution ofMRdiclofenac sodium pellets containing 100mg active ingredient was evaluated under simulated pre- and postprandial conditions using USP Apparatus 3 (reciprocating cylinder, Bio-Dis) and 4 (flow-through cell) and results compared with compendial methods using USP Apparatus 1 (basket) and 2 (paddle).
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Matrix Attachment Regions (MARs) are Thought to Buffer Transgenes
Plant Biotechnology Journal: Matrix attachment regions (MARs) are thought to buffer transgenes from the influence of surrounding chromosomal sequences, and therefore to reduce transgene silencing and variation in expression. The statistical properties of more than 400 independent transgenic events produced in Populus. The results are variable effects of MARs on expression level, variance and stability over time; Effect of MARs on correlated expression of linked GFP and BAR transgenes; effect of MARs on transgene integration and structure.
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Similarity of molecular phenotype between known epilepsy gene LGI1 and disease candidate gene LGI2
Bio Chemistry Research Journal : Similarity of molecular phenotype between known epilepsy gene LGI1 and disease candidate gene LGI2 This similarity of experimental mislocalization phenotypes for mutations at homologous positions of LGI2 and the established epilepsy gene LGI1 suggests that both genes share a potentially common molecular pathogenesis mechanism that might be the reason for genotypically distinct but phenotypically related forms of epilepsy. In our previous work, arguments based on protein sequence analysis and patient-specific chromosomal deletions are provided for LGI2 as the prime candidate gene for partial epilepsy with pericentral spikes (PEPS) [14]. Both PEPS and ADLTE are temporal lobe epilepsies and LGI1 and LGI2 are closely related paralogues that share the same functional domains.
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